Development of an Optimized Culture System for Generating Mouse Alveolar Macrophage–like Cells

Abstract Alveolar macrophages (AMs) play critical roles in maintaining lung homeostasis and orchestrating the immune responses. Although essential factors known for AM development have been identified, currently an optimal vitro culture system that can be used studying functions of AMs is still lacking. In this study, we report optimized generating AM-like cells from adult mouse bone marrow fetal liver on presence a combination GM-CSF, TGF-?, peroxisome proliferator–activated receptor ? (PPAR-?) agonist rosiglitazone. These expressed typical surface markers sialic acid–binding Ig-like lectin-F (Siglec-F), CD11c, F4/80, AM-specific genes, including carbonic anhydrase 4 (Car4), placenta-expressed transcript 1 (Plet1), eosinophil-associated RNase A family member (Ear1), cell death–inducing DNA fragmentation factor A–like effector c (Cidec), cytokeratin 19 (Krt19). Similar to primary AMs, alternative macrophage activation signature genes self-renewal genes. Moreover, could expansion bronchoalveolar lavage fluid–derived vitro. The generated resembled expanded inflammatory responses phagocytic activity. More importantly, these obtained sufficient numbers allowed genetic manipulation functional analysis Taken together, provide powerful tool biology AMs.